special stains in histopathology pdf

Leica Biosystems can clarify your organizations path to digitally driven discoveries or diagnostics. This autonomic ganglion from the myenteric plexus, located between the smooth muscle layers of the muscularis externa of the small intestine, contains ganglionic neurons that show well-defined basophilic Nissl substance (aggregations of endoplasmic reticulum and ribosomal RNA) in their cytoplasm. Leica Biosystems provides complete access to today's hottest topics in life sciences and in tissue-based translational research. Figure 10: Periodic Acid Schiff (kidney). Just following the method and not really knowing what should be seen in the finished section will lead to poor results. (PDF) Special Stains | Prof. Hesham N Mustafa - Academia.edu Get Knowledge Pathway updates delivered directly to your inbox. Others have to be left for some time to oxidize (ripen) before they can be used at all. The reticulin stain is useful in parenchymal organs such as liver and spleen to outline the architecture. ANNOTATED BIBLIOGRAPHY 1555-1995 APPENDED AS ANNEX. Document any departure from the method you are using. SPECIAL STAINS AND ITS APPLICATIONS IN Use of special stains for tissues not only aids in distinguishing structural alterations of tissues but also alerts the physician to alterations in tissue function highly relevant to making a diagnosis, such as abnormal deposition or iron, abnormal deposition of protein [amyloidosis, paraproteinemia, etc., abnormal accumulation of glycogen or 210-567-7000, Contact By colouring otherwise transparent tissue sections, these stains allow highly trained pathologists and researchers to view, under a microscope, tissue morphology (structure) or to look for the presence or prevalence of particular cell types, structures or even microorganisms such as bacteria. Table 9.1 Commonly used stain for different substances. Store reagents correctly. Deer Park, IL 60010 United States, Receive exclusive news, resources and special offers from Leica Biosystems. Introduction to Histology Stains | histology - University of Michigan Send us a submission and well be in touch! WebStaining Procedures: Each of the following .PDF files contains the procedure for a Know what you are trying to demonstrate with the stain you are performing. 0000013485 00000 n Special stains are used to visualize various tissue elements and An Intro to Routine and Special Staining in Histopathology It requires a formalin fixation. There are a wide variety of special stains to demonstrate pathologic processes. This helps to determine the pattern of tissue injury. These liver sections were stained by the same method. Copyright 2023 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. 0000001116 00000 n Special Stains 0000006042 00000 n Special Stains in Histopathological Techniques Figure 16: Gomori Trichrome (green) (submucosa). ^/%:2VZjnyU#=^ Use microscopic control at crucial stages such as differentiation steps. We are looking for more great writers to feature here. Hematoxylin is extracted from the logwood tree and purified. He is a former Senior Lecturer in histopathology in the Department of Laboratory Medicine, RMIT University in Melbourne, Australia. The slides are then dried in an oven or on a hot plate to remove moisture and help the tissue adhere to the slide. 0000016994 00000 n Anatomic Pathology Special Stains For the use of any product, the applicable product documentation, including information guides, inserts and operation manuals should be consulted. 0000015056 00000 n A: Wet section (no coverslip) viewed under a microscope with closed condenser diaphragm. ?.\ QT[y1~FSH 7O(/XIok-6XMCIxa)EX:){}AjPq Figure 14: Alcian Blue and PAS (intestine). Figure 15: Gomori Trichrome (blue) (submucosa). California: Do Not Sell or Share My Data. The reticulin fibers are black and better defined in section A (Gordon & Sweets method). This stain is used to detect and identify acid fast bacilli in tissue. Section A was treated with periodic acid (oxidation step) for 5 minutes where as section B had only 30 seconds (a mistake). Note the lack of differential coloration of muscle in section B (intestine). Section A was treated with periodic acid (oxidation step) for 5 minutes where as section B had only 30 seconds (a mistake). This information is often sufficient to allow a disease diagnosis based on the organization (or disorganization) of the cells and also shows any abnormalities or particular indicators in the actual cells (such as nuclear changes typically seen in cancer). HTK1)|19@H^`rEJ= . A comprehensive range of probes, detection, ancillaries, and instruments for automated or manual ISH detection in fluorescence and brightfield applications. In this case, the stain was performed correctly following the lab protocol and including a preliminary chromic acid step (sensitization or secondary mordanting). The only difference between them was the technique by which they were rinsed between impregnation and reduction.

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